An attempt to design an isoluminol-hydrogen peroxidase-amplified CL that measures intracellularly produced H2O2 in phagocytes: sensitivity for H2O2 is not high enough to allow detection

Luminescence. 2007 Sep-Oct;22(5):507-10. doi: 10.1002/bio.990.

Abstract

A technique was designed for the determination of hydrogen peroxide release. We found, however, that the isoluminol-amplified chemiluminescence technique is a suitable tool for measuring secretion of superoxide but not hydrogen peroxide.

MeSH terms

  • Cell Membrane / chemistry
  • Cell Membrane / metabolism
  • Equipment Design
  • Horseradish Peroxidase / chemistry*
  • Horseradish Peroxidase / metabolism
  • Hydrogen Peroxide* / analysis
  • Hydrogen Peroxide* / metabolism
  • Intracellular Fluid / chemistry
  • Intracellular Fluid / metabolism
  • Luminescent Measurements* / instrumentation
  • Luminescent Measurements* / methods
  • Luminol / analogs & derivatives*
  • Luminol / chemistry
  • Phagocytes / chemistry
  • Phagocytes / metabolism*
  • Sensitivity and Specificity
  • Superoxide Dismutase / chemistry*
  • Superoxide Dismutase / metabolism

Substances

  • isoluminol
  • Luminol
  • Hydrogen Peroxide
  • Horseradish Peroxidase
  • Superoxide Dismutase