Abstract
We have cloned, expressed, purified and characterised ceFKB-6, the only large tetratricopeptide repeat motif-containing immunophilin in Caenorhabditis elegans which is similar to the human orthologues FKBP51 and FKBP52. It shows increased peptidyl prolyl isomerase activity, the measured k(cat)/K(m) of 1.3 x 10(6) M(-1) s(-1)is twofold greater than that of hFKBP12 and hFKBP51. NMR studies of the interaction between FKB-6 and the C-terminal DAF-21 pentapeptide MEEVD show interactions consistent with those found between the large human immunophilin TPR domains and human Hsp90. In vivo localisation studies show that the fkb-6 gene is expressed in all stages from embryo to adult with predominant expression being noted in the adult dorsal and ventral nerve cords.
MeSH terms
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Animals
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans Proteins / biosynthesis
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Caenorhabditis elegans Proteins / chemistry
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Caenorhabditis elegans Proteins / genetics*
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Caenorhabditis elegans Proteins / metabolism
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Cattle
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Cloning, Molecular
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Cyclophilins / metabolism
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HSP90 Heat-Shock Proteins / chemistry
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HSP90 Heat-Shock Proteins / genetics
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HSP90 Heat-Shock Proteins / metabolism
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Immunophilins / biosynthesis
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Immunophilins / chemistry
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Immunophilins / genetics*
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Immunophilins / metabolism
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Magnetic Resonance Spectroscopy
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Models, Molecular
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Protein Binding
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Protein Conformation
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Protein Structure, Tertiary
Substances
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Caenorhabditis elegans Proteins
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DAF-21 protein, C elegans
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HSP90 Heat-Shock Proteins
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Cyclophilins
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FKB-6 protein, C elegans
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Immunophilins
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PPID protein, human