The Streptomyces coelicolor dnaK operon contains a second promoter driving the expression of the negative regulator hspR at physiological temperature

Arch Microbiol. 2007 Nov;188(5):541-6. doi: 10.1007/s00203-007-0269-y. Epub 2007 Jul 5.

Abstract

HspR (heat shock protein regulator) acts as a negative regulator of different genes in many bacteria. In Streptomyces coelicolor hspR gene is part and the transcriptional repressor of the dnaK operon which encodes the DnaK, GrpE, DnaJ chaperone machines and HspR itself. Our experiments led us to the discovery of a second promoter, internal to dnaK operon, located upstream hspR gene. Transcription from this promoter was detected at 30 degrees C indicating that hspR could play a key physiological role.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Base Sequence
  • Gene Expression Regulation, Bacterial*
  • HSP70 Heat-Shock Proteins / genetics
  • HSP70 Heat-Shock Proteins / metabolism*
  • Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / metabolism*
  • Molecular Sequence Data
  • Operon*
  • Promoter Regions, Genetic / genetics*
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Streptomyces coelicolor / genetics
  • Streptomyces coelicolor / metabolism*
  • Temperature

Substances

  • Bacterial Proteins
  • HSP70 Heat-Shock Proteins
  • Heat-Shock Proteins
  • HspR protein, bacteria
  • Repressor Proteins