Characterization of Fusarium graminearum inhibitory lipopeptide from Bacillus subtilis IB

Appl Microbiol Biotechnol. 2007 Sep;76(4):889-94. doi: 10.1007/s00253-007-1054-1. Epub 2007 Jul 5.


Bacillus subtilis strain IB exhibiting inhibitory activity against the Fusarium head blight disease fungus Fusarium graminearum was isolated and identified. The major inhibitory compound was purified from the culture broth through anion exchange, hydrophobic interaction, and reverse phase high-performance liquid chromatography (RP-HPLC) steps. It was a 1,463-Da lipopeptide and had an amino acid composition consisting of Ala, Glx, Ile, Orn, Pro, Thr, and Tyr at a molar ratio of 1:3:1:1:1:1:2. Electrospray ionization mass spectrometry/mass spectrometry (ESI MS/MS) analyses of the natural and the ring-opened peptides showed the antagonist was fengycin, a kind of macrolactone molecule with antifungal activity produced by several Bacillus strains. Fluorescence microscopic analysis indicated this peptide permeabilized and disrupted F. graminearum hyphae.

MeSH terms

  • Amino Acids / analysis
  • Antifungal Agents / chemistry*
  • Antifungal Agents / isolation & purification
  • Antifungal Agents / pharmacology*
  • Bacillus subtilis / chemistry*
  • Bacillus subtilis / metabolism
  • Chromatography, Ion Exchange
  • Electrophoresis, Polyacrylamide Gel
  • Fusarium / drug effects*
  • Fusarium / growth & development
  • Lipopeptides
  • Lipoproteins / chemistry
  • Lipoproteins / isolation & purification
  • Lipoproteins / pharmacology
  • Microscopy, Fluorescence
  • Spectrometry, Mass, Electrospray Ionization


  • Amino Acids
  • Antifungal Agents
  • Lipopeptides
  • Lipoproteins
  • fengycin