beta-Amyloid deposition and prion infection in adult primary brain cell long-term culture model

Biochem Biophys Res Commun. 2007 Aug 31;360(3):520-4. doi: 10.1016/j.bbrc.2007.05.227. Epub 2007 Jun 26.


We report an in vitro model of the adult central nervous system produced by culturing primary brain cells isolated from adult mice for periods longer than 4 months. We applied this novel cell culture method to model progressive neurodegenerative diseases. After long-term culture of adult primary brain cells prepared from Alzheimer's disease and prion disease mouse models, we observed beta-amyloid deposition and prion infection in primary cell cultures in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid beta-Peptides / metabolism*
  • Animals
  • Brain / metabolism*
  • Brain / pathology*
  • Cells, Cultured
  • Disease Progression
  • Mice
  • Mice, Inbred C57BL
  • Models, Biological
  • Prion Diseases / metabolism*
  • Prion Diseases / pathology*
  • Survival Rate
  • Time Factors


  • Amyloid beta-Peptides