Rcs and PhoPQ regulatory overlap in the control of Salmonella enterica virulence

J Bacteriol. 2007 Sep;189(18):6635-44. doi: 10.1128/JB.00640-07. Epub 2007 Jul 6.


Genetic screens based on the use of MudJ-generated lac fusions permitted the identification of novel genes regulated by the Rcs signal transduction system in Salmonella enterica serovar Typhimurium. Besides genes that are also found in the Escherichia coli genome, our screens identified Salmonella-specific genes regulated by RcsB, including bapA, siiE, srfA, and srfB. Here we show that the srfABC operon is negatively regulated by RcsB and by PhoP. In vivo studies using mutants with constitutive activation of the Rcs and/or PhoPQ system suggested that there is an overlap between these regulatory systems in the control of Salmonella virulence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Female
  • Gene Expression Regulation, Bacterial*
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Salmonella Infections, Animal / microbiology
  • Salmonella typhimurium / genetics
  • Salmonella typhimurium / metabolism
  • Salmonella typhimurium / pathogenicity*
  • Salmonella typhimurium / physiology
  • Signal Transduction*
  • Virulence


  • Bacterial Proteins
  • PhoP protein, Bacteria
  • RcsB protein, Bacteria