Comprehensive analysis of proteins secreted by Trichophyton rubrum and Trichophyton violaceum under in vitro conditions

J Proteome Res. 2007 Aug;6(8):3081-92. doi: 10.1021/pr070153m. Epub 2007 Jul 10.


Dermatophytes cause most superficial mycoses in humans and animals. Their pathogenicity is probably linked with the secretion of proteins degrading keratinised structures. Using 2D-PAGE and a shotgun mass spectrometry approach, we identified 80 proteins from Trichophyton rubrum and Trichophyton violaceum secretomes, under conditions mimicking those in the host. Identified proteins included endo- and exoproteases, other hydrolases, and oxidoreductases. Our findings can contribute to a better understanding of the virulence mechanisms of the two species and the different types of infection they cause.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Gel, Two-Dimensional / methods
  • Enzymes / analysis*
  • Enzymes / metabolism
  • Fungal Proteins / analysis*
  • Fungal Proteins / metabolism
  • Humans
  • Mass Spectrometry / methods
  • Proteome / metabolism*
  • Trichophyton / enzymology*


  • Enzymes
  • Fungal Proteins
  • Proteome