Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues

J Gen Virol. 2007 Aug;88(Pt 8):2228-2236. doi: 10.1099/vir.0.82757-0.


We have determined the high resolution crystal structure of the methyltransferase domain of the NS5 polypeptide from the Murray Valley encephalitis virus. This domain is unusual in having both the N7 and 2'-O methyltransferase activity required for Cap 1 synthesis. We have also determined structures for complexes of this domain with nucleotides and cap analogues providing information on cap binding, based on which we suggest a model of how the sequential methylation of the N7 and 2'-O groups of the cap may be coordinated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carcinoembryonic Antigen / metabolism
  • Crystallization
  • Encephalitis Virus, Murray Valley / enzymology*
  • Methylation
  • Methyltransferases / chemistry*
  • Methyltransferases / genetics
  • Methyltransferases / metabolism
  • Models, Molecular
  • Molecular Sequence Data
  • Oligopeptides / metabolism
  • Protein Structure, Tertiary / genetics
  • RNA Cap Analogs / metabolism*
  • Sequence Alignment
  • Viral Nonstructural Proteins / chemistry*
  • Viral Nonstructural Proteins / genetics
  • Viral Nonstructural Proteins / metabolism


  • CAP1-6D
  • Carcinoembryonic Antigen
  • NS5 protein, flavivirus
  • Oligopeptides
  • RNA Cap Analogs
  • Viral Nonstructural Proteins
  • Methyltransferases

Associated data

  • PDB/2PX2
  • PDB/2PX4
  • PDB/2PX5
  • PDB/2PX8
  • PDB/2PXA
  • PDB/2PXC