DNA architectural factor and proto-oncogene HMGA2 regulates key developmental genes in pluripotent human embryonic stem cells

FEBS Lett. 2007 Jul 24;581(18):3533-7. doi: 10.1016/j.febslet.2007.06.072. Epub 2007 Jul 5.

Abstract

The high-mobility group (HMG) protein A2 has been studied mostly in the mouse where its function seems critical for embryonic cell growth and adipogenesis, leading to a pygmy phenotype with greatly reduced fat tissue in homozygous knock out mice. We showed recently that among the major HMG proteins, HMGA2 is highly expressed in two human embryonic stem (hES) cell lines. Here, we employed siRNA technology in combination with quantitative reverse transcriptase polymerase chain reaction, stem cell-specific microarray analyses, and cell proliferation assays in order to probe into HMGA2's role(s) in pluripotent hES cells. Our results establish HMGA2 as a regulator of human genes linked to mesenchymal cell differentiation, adipogenesis, and hES cell growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology
  • Adipocytes / metabolism
  • Biomarkers
  • Cell Differentiation
  • Cell Line
  • Cell Proliferation
  • DNA / genetics*
  • Down-Regulation
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / metabolism*
  • Gene Expression Regulation, Developmental / genetics*
  • HMGA2 Protein / genetics
  • HMGA2 Protein / metabolism*
  • Humans
  • RNA, Small Interfering / genetics

Substances

  • Biomarkers
  • HMGA2 Protein
  • RNA, Small Interfering
  • DNA