Induction of cytotoxic T cell response against HCA661 positive cancer cells through activation with novel HLA-A *0201 restricted epitopes

Cancer Lett. 2007 Oct 28;256(2):178-85. doi: 10.1016/j.canlet.2007.06.002. Epub 2007 Jul 10.

Abstract

HCA661 is a cancer-testis (CT) antigen frequently expressed in human hepatocellular carcinoma (HCC). To search for immunogenic peptides of HCA661, bioinformatics analysis and CD8(+) T cell IFN-gamma ELISPOT assay were employed, and two HLA-A *0201 restricted peptides, H110 and H246, were identified. These two HCA661 peptides are naturally processed in dendritic cells (DCs) and when used for DCs loading, they are sufficient to prime autologous CD8(+) T cells to elicit cytotoxic response against HCA661(+) human cancer cells. The HCA661 peptides, H110 and H246, are hence attractive candidates for human cancer immunotherapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / immunology*
  • Carcinoma, Hepatocellular / immunology*
  • Carcinoma, Hepatocellular / pathology
  • Carcinoma, Hepatocellular / therapy
  • Cell Adhesion Molecules, Neuronal / immunology*
  • Cell Line, Tumor
  • Cell Survival
  • Computational Biology
  • Enzyme-Linked Immunosorbent Assay
  • Epitope Mapping / methods
  • Feasibility Studies
  • Fetal Proteins / immunology*
  • HLA-A Antigens / immunology*
  • HLA-A2 Antigen
  • Humans
  • Immunodominant Epitopes*
  • Immunotherapy / methods
  • Interferon-gamma / metabolism
  • Liver Neoplasms / immunology*
  • Liver Neoplasms / pathology
  • Lymphocyte Activation*
  • T-Lymphocytes, Cytotoxic / immunology*
  • T-Lymphocytes, Cytotoxic / metabolism

Substances

  • ALCAM protein, human
  • Antigens, CD
  • Cell Adhesion Molecules, Neuronal
  • Fetal Proteins
  • HLA-A Antigens
  • HLA-A*02:01 antigen
  • HLA-A2 Antigen
  • Immunodominant Epitopes
  • Interferon-gamma