Rab11-FIP2 regulates differentiable steps in transcytosis

Am J Physiol Cell Physiol. 2007 Sep;293(3):C1059-72. doi: 10.1152/ajpcell.00078.2007. Epub 2007 Jul 11.


Transcytosis through the apical recycling system of polarized cells is regulated by Rab11a and a series of Rab11a-interacting proteins. We have identified a point mutant in Rab11 family interacting protein 2 (Rab11-FIP2) that alters the function of Rab11a-containing trafficking systems. Rab11-FIP2(S229A/R413G) or Rab11-FIP2(R413G) cause the formation of a tubular cisternal structure containing Rab11a and decrease the rate of polymeric IgA transcytosis. The R413G mutation does not alter Rab11-FIP interactions with any known binding partners. Overexpression of Rab11-FIP2(S229A/R413G) alters the localization of a subpopulation of the apical membrane protein GP135. In contrast, Rab11-FIP2(129-512) alters the localization of early endosome protein EEA1. The distributions of both Rab11-FIP2(S229A/R413G) and Rab11-FIP2(129-512) were not dependent on the integrity of the microtubule cytoskeleton. The results indicate that Rab11-FIP2 regulates trafficking at multiple points within the apical recycling system of polarized cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism*
  • Cell Line
  • Cell Polarity / physiology
  • Dogs
  • Endosomes / metabolism
  • Epithelial Cells / cytology
  • Epithelial Cells / metabolism*
  • Genes, Dominant
  • Green Fluorescent Proteins / genetics
  • Humans
  • Kidney Tubules / cytology
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism*
  • Microscopy, Electron
  • Microtubules / metabolism
  • Microtubules / ultrastructure
  • Mutagenesis, Site-Directed
  • Point Mutation
  • Protein Transport / physiology*
  • Vesicular Transport Proteins / metabolism
  • rab GTP-Binding Proteins


  • Carrier Proteins
  • EHD1 protein, human
  • EHD3 protein, human
  • Membrane Proteins
  • Vesicular Transport Proteins
  • Green Fluorescent Proteins
  • Rab11-FIP2 protein, human
  • rab GTP-Binding Proteins