Assessing enzyme activities using stable isotope labeling and mass spectrometry

Mol Cell Proteomics. 2007 Oct;6(10):1771-7. doi: 10.1074/mcp.M700057-MCP200. Epub 2007 Jul 11.


Activity-based protein profiling has emerged as a valuable technology for labeling, enriching, and assessing protein activities from complex mixtures. This is primarily accomplished via a two-step identification and quantification process. Here we show a highly quantitative and streamlined method, termed catch-and-release activity profiling of enzymes (CAPE), which reduces this procedure to a single step. Furthermore the CAPE approach has the ability to detect small quantitative changes that may have been missed by alternative mass spectrometry-based techniques.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cattle
  • Cell Line, Tumor
  • Humans
  • Isotope Labeling / methods*
  • Mass Spectrometry*
  • Molecular Sequence Data
  • Neoplasm Metastasis
  • Neoplasm Proteins / chemistry
  • Neoplasm Proteins / metabolism
  • Organophosphonates / metabolism
  • Peptides / chemistry
  • Peptides / metabolism
  • Trypsin / metabolism*
  • Trypsinogen / metabolism*


  • Neoplasm Proteins
  • Organophosphonates
  • Peptides
  • Trypsinogen
  • Trypsin