Identification and characterization of a novel alternative splice variant of mouse GMx33alpha/GPP34

Gene. 2007 Oct 1;400(1-2):82-8. doi: 10.1016/j.gene.2007.06.007. Epub 2007 Jun 19.

Abstract

We have cloned and characterized a novel splice variant of mouse GMx33alpha/Golgi-associated protein of 34 kDa (GPP34), hereby designated GMx33alphaV/GPP34V. This splice variant skips the second and third exons, and the resulting frame shift generates a stop codon in the fourth exon. GMx33alphaV/GPP34V is comprised of 81 amino acid residues derived from the N-terminal end of the full length protein and corresponds to approximately one-third of the full length GMx33alpha/GPP34 sequence with a calculated molecular mass of 8900. In contrast to GMx33alpha/GPP34 mRNA which is expressed at similar levels in various tissues, GMx33alphaV/GPP34V mRNA was differentially expressed when examined by RT-PCR. Compared to other tissues, skeletal muscle showed relatively strong expression of GMx33alphaV/GPP34V mRNA. This splice variant cDNA was also detected in a human cell line.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing*
  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Fluorescent Antibody Technique
  • HeLa Cells
  • Humans
  • Membrane Proteins / analysis
  • Membrane Proteins / genetics
  • Mice
  • Molecular Sequence Data
  • Muscle, Skeletal / metabolism
  • Phosphoproteins / chemistry
  • Phosphoproteins / genetics*
  • Protein Isoforms / genetics*
  • RNA, Messenger / metabolism
  • Sequence Alignment
  • Transfection

Substances

  • GOLPH3 protein, human
  • GPP34 protein, mouse
  • Membrane Proteins
  • Phosphoproteins
  • Protein Isoforms
  • RNA, Messenger