Quantitative determination of salidroside in rat plasma by on-line solid-phase extraction integrated with high-performance liquid chromatography/electrospray ionization tandem mass spectrometry

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Sep 15;857(1):164-9. doi: 10.1016/j.jchromb.2007.06.029. Epub 2007 Jul 1.


A method for the quantification of salidroside, a major biologically active compound in Rhodiola, in rat plasma by on-line SPE LC/MS/MS in negative electrospray mode was developed and validated. A column-switching instrument and two HPLC pumping systems were employed, and salicin was used as the internal standard. A Waters Oasis HLB extraction column and an Agilent TC-C(18) analytical column in a column-switching set-up with gradient elution were utilized. The MS/MS ion transitions monitored were m/z 299.0/119.0 and 285.1/122.9 for salidroside and salicin, respectively. The standard curves were linear within a range of 50-5000 ng/mL using weighted linear regression analysis (1/x). The intra- and inter-day coefficients of variance ranged from 1% to 9%. The recovery was above 90%. The freeze/thaw and long-term stability were validated. This method was subsequently applied to a pharmacokinetic study of salidroside in rats.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Administration, Oral
  • Animals
  • Benzyl Alcohols / standards
  • Calibration
  • Chromatography, High Pressure Liquid / methods*
  • Drug Stability
  • Drugs, Chinese Herbal / pharmacokinetics
  • Glucosides / administration & dosage
  • Glucosides / blood*
  • Glucosides / pharmacokinetics
  • Injections, Intravenous
  • Male
  • Online Systems
  • Phenols / administration & dosage
  • Phenols / blood*
  • Phenols / pharmacokinetics
  • Rats
  • Rats, Sprague-Dawley
  • Reference Standards
  • Rhodiola / chemistry
  • Sensitivity and Specificity
  • Solid Phase Extraction / methods*
  • Spectrometry, Mass, Electrospray Ionization / methods
  • Tandem Mass Spectrometry / methods


  • Benzyl Alcohols
  • Drugs, Chinese Herbal
  • Glucosides
  • Phenols
  • salicin
  • rhodioloside