Clinical phenotypes associated with the complement factor H Y402H variant in age-related macular degeneration

Am J Ophthalmol. 2007 Sep;144(3):404-408. doi: 10.1016/j.ajo.2007.05.018. Epub 2007 Jul 12.

Abstract

Purpose: To determine whether the complement factor H (CFH) Y402H variant is associated with specific age-related macular degeneration (AMD) clinical phenotypes.

Design: Retrospective, case-control study.

Methods: One hundred and eighty-eight white subjects with AMD and 189 control subjects were genotyped for the T-to-C polymorphism in exon 9 of the CFH gene by restriction-fragment length analysis and deoxyribonucleic acid (DNA) sequencing using genomic DNA from mouthwash samples. AMD phenotypes were characterized by clinical examination, fundus photography, and fluorescein angiography.

Results: Heterozygosity for the at-risk genotype (TC) increased the likelihood for AMD 2.1-fold (95% confidence interval [CI], 1.3 to 3.3), whereas homozygosity for the genotype (CC) increased the likelihood for AMD 6.5-fold (95% CI, 3.4 to 12.5) in our population. The C allele was associated significantly with predominantly classic choroidal neovascularization (odds ratio [OR], 2.01; 95% CI, 1.34 to 3.30). Neovascular lesion size was similar among the three genotypes (P = .67).

Conclusions: The Y402H CFH variant carried a significantly increased risk for developing AMD in our population. Genotype and phenotype correlations regarding choroidal neovascular lesion type were observed.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Aged
  • Case-Control Studies
  • Choroidal Neovascularization / diagnosis
  • Choroidal Neovascularization / genetics
  • Complement Factor H / genetics
  • Exons / genetics
  • Female
  • Fluorescein Angiography
  • Genetic Variation*
  • Genotype
  • Humans
  • Macular Degeneration / diagnosis
  • Macular Degeneration / genetics*
  • Male
  • Phenotype
  • Polymorphism, Restriction Fragment Length
  • Polymorphism, Single Nucleotide*
  • Retrospective Studies
  • Sequence Analysis, DNA

Substances

  • CFH protein, human
  • Complement Factor H