Objective: To isolate and identify pronuciferine monomer.
Methods: The primary compositions in the seed of Nelumbo nucifera were extracted by supercritical CO2 way, then isolated and purified by column chroatography fractionation and identified by HPLC, TLC, GC-MS.
Results: A monomer was further isolated and purifed by column chromatography fractionation from the primary compositions in the seed of Nelumbo nucifera which were extracted by supercritical CO2 way. The compositions of the monomer was pronuciferine which wrer identified by HPLC, TLC, GC-MS.
Conclusion: The pronucierine monomer could be isolated from the seed of Nelumbo nucifera by combining supercitical CO2 way with column chromatography fractionation, which will be available for further functional study.