[Inhibitory effects of cordyceps extract on growth of colon cancer cells]

Zhong Yao Cai. 2007 Mar;30(3):310-3.
[Article in Chinese]

Abstract

Objective: To observe the inhibiting effect of Cordyceps extract on the growth of colon cancer cell lines in vitro and its mechanism.

Methods: MTT assay was used to evaluate the inhibiting effect of Cordyceps extract on the proliferation of colon cancer cells. Report Gene was used to evaluate the relative NF-kappaB activity of the SW480 cells which were transfectd by pNF-kappaB-TA-Luc report gene. Western blot analysis was used to evaluate the content of IkappaBalpha in SW480 cells.

Results: Cordyceps extract could inhibit the proliferation of HT-29 and SW480 cells in a dose-dependent manner. Report gene showed that TNF-alpha enhance the activation of NF-kappaB in SW480 cells and Cordyceps extract Could decrease the activation of NF-kappaB induced by TNF-alpha in SW480 cells. Western blot analysis showed that TNF-alpha can induce the degradation of IkappaBalpha of SW480 cells and Cordyceps extract can inhibit it.

Conclusion: Cordyceps extract can inhibit the proliferation of colon cancer cells through inhibiting the degradation of lkappaBalpha in the cell and inhibiting the activation of NF-kappaB.

MeSH terms

  • Apoptosis / drug effects
  • Cell Line, Tumor
  • Cell Proliferation / drug effects*
  • Colonic Neoplasms / metabolism
  • Colonic Neoplasms / pathology*
  • Cordyceps / chemistry*
  • Humans
  • I-kappa B Proteins
  • NF-KappaB Inhibitor alpha
  • NF-kappa B
  • Tumor Necrosis Factor-alpha

Substances

  • I-kappa B Proteins
  • NF-kappa B
  • NFKBIA protein, human
  • Tumor Necrosis Factor-alpha
  • NF-KappaB Inhibitor alpha