Abeta oligomer-mediated long-term potentiation impairment involves protein phosphatase 1-dependent mechanisms

J Neurosci. 2007 Jul 18;27(29):7648-53. doi: 10.1523/JNEUROSCI.0395-07.2007.


Amyloid beta (Abeta) oligomers are derived from proteolytic cleavage of amyloid precursor protein (APP) and can impair memory and hippocampal long-term potentiation (LTP) in vivo and in vitro. They are recognized as the primary neurotoxic agents in Alzheimer's disease. The mechanisms underlying such toxicity on synaptic functions are complex and not fully understood. Here, we provide the first evidence that these mechanisms involve protein phosphatase 1 (PP1). Using a novel transgenic mouse model expressing human APP with the Swedish and Arctic mutations that render Abeta more prone to form oligomers (arcAbeta mice), we show that the LTP impairment induced by Abeta oligomers can be fully reversed by PP1 inhibition in vitro. We further demonstrate that the genetic inhibition of endogenous PP1 in vivo confers resistance to Abeta oligomer-mediated toxicity and preserves LTP. Overall, these results reveal that PP1 is a key player in the mechanisms of AD pathology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Amyloid Precursor Protein Secretases / genetics
  • Amyloid beta-Peptides / chemistry
  • Amyloid beta-Peptides / metabolism*
  • Amyloid beta-Peptides / ultrastructure
  • Analysis of Variance
  • Animals
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics
  • Dose-Response Relationship, Radiation
  • Electric Stimulation / methods
  • Excitatory Postsynaptic Potentials / drug effects
  • Excitatory Postsynaptic Potentials / physiology
  • Gene Expression Regulation / genetics
  • Hippocampus / cytology
  • Humans
  • In Vitro Techniques
  • Long-Term Potentiation / genetics
  • Long-Term Potentiation / physiology*
  • Long-Term Potentiation / radiation effects
  • Mice
  • Mice, Transgenic
  • Microscopy, Electron, Transmission / methods
  • Neurons / drug effects
  • Neurons / physiology*
  • Patch-Clamp Techniques
  • Phosphoprotein Phosphatases / physiology*
  • Presenilin-1 / genetics
  • Protein Phosphatase 1
  • Reverse Transcriptase Polymerase Chain Reaction / methods


  • Amyloid beta-Peptides
  • Presenilin-1
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 1
  • Amyloid Precursor Protein Secretases