Physiologically Distinct Temporal Cohorts of Cortical Interneurons Arise From Telencephalic Olig2-expressing Precursors

J Neurosci. 2007 Jul 18;27(29):7786-98. doi: 10.1523/JNEUROSCI.1807-07.2007.

Abstract

Inhibitory GABAergic interneurons of the mouse neocortex are a highly heterogeneous population of neurons that originate from the ventral telencephalon and migrate tangentially up into the developing cortical plate. The majority of cortical interneurons arise from a transient embryonic structure known as the medial ganglionic eminence (MGE), but how the remarkable diversity is specified in this region is not known. We have taken a genetic fate mapping strategy to elucidate the temporal origins of cortical interneuron subtypes within the MGE. We used an inducible form of Cre under the regulation of Olig2, a basic helix-loop-helix transcription factor highly expressed in neural progenitors of the MGE. We observe that the physiological subtypes of cortical interneurons are, to a large degree, unique to their time point of generation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / physiology
  • Age Factors
  • Animals
  • Animals, Newborn
  • Basic Helix-Loop-Helix Transcription Factors / genetics
  • Basic Helix-Loop-Helix Transcription Factors / metabolism*
  • Body Patterning / physiology
  • Cell Differentiation / genetics
  • Cell Differentiation / physiology
  • Cell Movement / genetics
  • Cell Movement / physiology*
  • Cerebral Cortex / cytology*
  • Cerebral Cortex / embryology*
  • Embryo, Mammalian
  • Gene Expression Regulation, Developmental / physiology
  • Green Fluorescent Proteins / metabolism
  • In Vitro Techniques
  • Interneurons / classification
  • Interneurons / physiology*
  • Mice
  • Mice, Transgenic
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Oligodendrocyte Transcription Factor 2
  • Patch-Clamp Techniques / methods
  • Stem Cells / physiology*
  • Telencephalon / cytology

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • Nerve Tissue Proteins
  • Olig2 protein, mouse
  • Oligodendrocyte Transcription Factor 2
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins