JAZ repressor proteins are targets of the SCF(COI1) complex during jasmonate signalling

Nature. 2007 Aug 9;448(7154):661-5. doi: 10.1038/nature05960. Epub 2007 Jul 18.


Jasmonate and related signalling compounds have a crucial role in both host immunity and development in plants, but the molecular details of the signalling mechanism are poorly understood. Here we identify members of the jasmonate ZIM-domain (JAZ) protein family as key regulators of jasmonate signalling. JAZ1 protein acts to repress transcription of jasmonate-responsive genes. Jasmonate treatment causes JAZ1 degradation and this degradation is dependent on activities of the SCF(COI1) ubiquitin ligase and the 26S proteasome. Furthermore, the jasmonoyl-isoleucine (JA-Ile) conjugate, but not other jasmonate-derivatives such as jasmonate, 12-oxo-phytodienoic acid, or methyl-jasmonate, promotes physical interaction between COI1 and JAZ1 proteins in the absence of other plant proteins. Our results suggest a model in which jasmonate ligands promote the binding of the SCF(COI1) ubiquitin ligase to and subsequent degradation of the JAZ1 repressor protein, and implicate the SCF(COI1)-JAZ1 protein complex as a site of perception of the plant hormone JA-Ile.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / drug effects*
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Cell-Free System
  • Cyclopentanes / pharmacology*
  • Genes, Plant / genetics
  • Glucuronidase / genetics
  • Glucuronidase / metabolism
  • Isoleucine / analogs & derivatives*
  • Isoleucine / pharmacology
  • Molecular Sequence Data
  • Multigene Family / genetics
  • Oxylipins
  • Phenotype
  • Protein Binding
  • Protein Structure, Tertiary
  • Repressor Proteins / chemistry
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • SKP Cullin F-Box Protein Ligases / metabolism*
  • Signal Transduction / drug effects*
  • Substrate Specificity


  • Arabidopsis Proteins
  • Cyclopentanes
  • Oxylipins
  • Repressor Proteins
  • jasmonoyl-isoleucine
  • Isoleucine
  • jasmonic acid
  • SKP Cullin F-Box Protein Ligases
  • Glucuronidase

Associated data

  • GENBANK/EF591123
  • GEO/GDS2133