Amino-terminal presequence of the precursor of peroxisomal 3-ketoacyl-CoA thiolase is a cleavable signal peptide for peroxisomal targeting

Biochem Biophys Res Commun. 1991 Dec 31;181(3):947-54. doi: 10.1016/0006-291x(91)92028-i.


To examine the function of the amino-terminal presequence of rat peroxisomal 3-ketoacyl-CoA thiolase precursor, fusion proteins of various amino-terminal regions of the precursor with non-peroxisomal enzymes were expressed in cultured mammalian cells. On immunofluorescence microscopy, all constructs carrying the presequence part exhibited punctate patterns of distribution, identical with that of catalase, a peroxisomal marker. Proteins lacking all or a part of the prepiece were found in the cytosol. These results indicate that the presequence of the thiolase has sufficient information for peroxisomal targeting.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyl-CoA C-Acyltransferase / genetics*
  • Acetyl-CoA C-Acyltransferase / metabolism
  • Amino Acid Sequence
  • Animals
  • CHO Cells
  • Cricetinae
  • Enzyme Precursors / genetics*
  • Enzyme Precursors / metabolism
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Fluorescent Antibody Technique
  • Humans
  • Microbodies / enzymology*
  • Molecular Sequence Data
  • Plants / enzymology
  • Plants / genetics
  • Plasmids
  • Protein Processing, Post-Translational*
  • Protein Sorting Signals / genetics*
  • Protein Sorting Signals / metabolism
  • Rats
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology, Nucleic Acid
  • Tetrahydrofolate Dehydrogenase / genetics
  • Tetrahydrofolate Dehydrogenase / metabolism
  • Transfection


  • Enzyme Precursors
  • Protein Sorting Signals
  • Recombinant Fusion Proteins
  • Tetrahydrofolate Dehydrogenase
  • Acetyl-CoA C-Acyltransferase