Lactobacillus Acidophilus strain L-92 regulates the production of Th1 cytokine as well as Th2 cytokines

Allergol Int. 2007 Sep;56(3):293-301. doi: 10.2332/allergolint.O-06-459. Epub 2007 Aug 1.


Background: There is growing interest in probiotics such as lactic acid bacteria (LAB), not only for treatment of T helper type (Th) 1-mediated diseases but also for Th2-mediated diseases, including allergic diseases, since lactic acid bacteria may be able to modulate the Th1/Th2 balance, in addition to having an immunomodulative effect through induction of Th1 bias.

Methods: The effect of oral administration of heat-killed Lactobacillus acidophilus Strain L-92 (L-92) on ovalbumin (OVA)-specific immunoglobulin (Ig)E production was investigated in BALB/c mice. L-92 was orally administered to mice for 8 weeks from 2 weeks after initiation of OVA-immunization. Patterns of cytokine and Ig production in splenocytes and cells from Peyer's patches (PPs) from these mice were examined after restimulation with OVA in vitro.

Results: L-92 significantly suppressed serum OVA-specific IgE levels for a long period. Cytokines such as interferon (IFN)-gamma, interleukin (IL)-4 and IL-10 and Igs such as total IgE and OVA-specific IgE were produced at significantly lower levels by splenocytes of L-92-treated mice, compared with those of control mice. In contrast, transforming growth factor (TGF)-beta and IgA levels produced by PPs from L-92-treated mice were significantly higher than in those from control mice.

Conclusions: Oral L-92 administration regulated both Th1 and Th2 cytokine responses, suppressed serum OVA-specific IgE, and induced TGF-beta production in PPs. TGF-beta is known to be associated with activation of regulatory T (Treg) cells. These data suggest that LAB may have immunomodulative effect by Treg cells via TGF-beta activity.

MeSH terms

  • Animals
  • Cells, Cultured
  • Immunoglobulin E / immunology*
  • Immunosuppression / methods*
  • Interferon-gamma / immunology
  • Interleukin-1 / immunology
  • Interleukin-10 / immunology
  • Lactobacillus acidophilus / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Ovalbumin / immunology*
  • Peyer's Patches / immunology
  • Spleen / immunology
  • T-Lymphocytes, Regulatory
  • Transforming Growth Factor beta / immunology


  • Interleukin-1
  • Transforming Growth Factor beta
  • Interleukin-10
  • Immunoglobulin E
  • Interferon-gamma
  • Ovalbumin