Alternatively spliced mRNA of the pX region of human T lymphotropic virus type I proviral genome

FEBS Lett. 1991 Dec 16;295(1-3):141-5. doi: 10.1016/0014-5793(91)81404-v.

Abstract

The pX region of human T lymphotropic virus type I (HTLV-I) is believed to be expressed as a consequence of a 2 step splicing. It is conceivable, however, that a donor site of the 1st splicing and an acceptor site of the 2nd splicing results in the production of an alternatively spliced mRNA which is capable of coding p21X-III. This possibility was examined by amplifying cDNA derived from HTLV-I+ cells between the 5' LTR and pX region. Bands of 2 different sizes were consistently observed. Sequencing of the longer band corresponded to a cDNA derived from a double-spliced pX mRNA as previously reported. The shorter band was derived from a single-spliced mRNA. HTLV-I+ cell lines had both mRNAs to a varying degree. Expression of p40tax and p21X-III seem to be well correlated with a double-spliced and a single-spliced mRNA, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • Blotting, Western
  • Cell Line
  • DNA, Viral / genetics
  • Genome, Viral*
  • Human T-lymphotropic virus 1 / genetics*
  • Humans
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Oligonucleotide Probes
  • Polymerase Chain Reaction / methods
  • Proviruses / genetics*
  • RNA Splicing*
  • RNA, Messenger / genetics*
  • Repetitive Sequences, Nucleic Acid
  • Retroviridae Proteins, Oncogenic / genetics*
  • Transcription Factors*
  • Viral Proteins / analysis
  • Viral Proteins / genetics
  • Viral Regulatory and Accessory Proteins

Substances

  • DNA, Viral
  • Oligodeoxyribonucleotides
  • Oligonucleotide Probes
  • RNA, Messenger
  • Retroviridae Proteins, Oncogenic
  • Transcription Factors
  • Viral Proteins
  • Viral Regulatory and Accessory Proteins
  • pX protein, Human T-lymphotropic virus 1