Xylose-specific antibodies as markers of subcompartmentation of terminal glycosylation in the Golgi apparatus of sycamore cells

FEBS Lett. 1991 Dec 16;295(1-3):179-84. doi: 10.1016/0014-5793(91)81413-3.

Abstract

Antibodies specific for xylose-containing plant complex N-linked glycans are used for indirect immunolocalization of xylosyltransferase in sycamore cells. The use of high pressure freezing and freeze substitution for sample preparation resulted in very good morphological preservation of the different Golgi cisternae. Xylosyltransferase shows a diffuse distribution all over the Golgi stacks and xylosylation appears to be an early processing event that is initiated in the cis Golgi compartment.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies* / ultrastructure
  • Biomarkers
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Cells, Cultured
  • Electrophoresis, Polyacrylamide Gel
  • Glycoproteins / analysis
  • Glycosylation
  • Golgi Apparatus / metabolism*
  • Microscopy, Immunoelectron
  • Molecular Sequence Data
  • Oligosaccharides / chemistry
  • Oligosaccharides / isolation & purification
  • Pentosyltransferases / analysis
  • Plants / metabolism*
  • Xylose / analysis*
  • Xylose / immunology

Substances

  • Antibodies
  • Biomarkers
  • Glycoproteins
  • Oligosaccharides
  • Xylose
  • Pentosyltransferases
  • UDP xylose-protein xylosyltransferase