Establishment of novel human dedifferentiated chondrosarcoma cell line with osteoblastic differentiation

Virchows Arch. 2007 Sep;451(3):691-9. doi: 10.1007/s00428-007-0426-3. Epub 2007 Jul 26.


Dedifferentiated chondrosarcoma is a rare, highly malignant variant of chondrosarcoma in which a high-grade sarcoma coexists with a low-grade chondroid tumor. We herein review a case of dedifferentiated chondrosarcoma with an osteosarcoma omit component that occurred in the distal femur of a 38-year-old man. We established the cell line (NDCS-1) from a pleural effusion of the metastatic lung tumor. The cell line was characterized by a the G-banded karyotype, polymerase chain reaction (PCR) single-strand conformation polymorphism analysis, spectral karyotyping, and reverse transcriptase PCR (RT-PCR). The tumor exhibited complex karyotypes and a high frequency of chromosomal amplication with p53 mutation. This tumor revealed an osteoblastic and chondroblastic character in vitro and in severe combined immunodeficiency mice. The expression and phosphorylation of platelet-derived growth factor receptor-beta, which seemed to play a major role in the malignant phenotype of chondrosarcoma, was confirmed by RT-PCR and Western blotting. To our knowledge, this is the first report of the establishment of a human dedifferentiated chondrosarcoma.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Animals
  • Blotting, Western
  • Cell Differentiation*
  • Cell Division
  • Cell Line, Tumor*
  • Chondrosarcoma / genetics
  • Chondrosarcoma / pathology*
  • Chromosome Aberrations
  • Femoral Neoplasms / genetics
  • Femoral Neoplasms / pathology*
  • Genes, p53 / genetics
  • Humans
  • Karyotyping
  • Lung Neoplasms / pathology
  • Lung Neoplasms / secondary
  • Male
  • Mice
  • Mice, SCID
  • Mutation
  • Neoplasm Transplantation
  • Osteoblasts / pathology*
  • Pleural Effusion / cytology
  • Polymorphism, Single-Stranded Conformational
  • Receptor, Platelet-Derived Growth Factor beta / genetics
  • Receptor, Platelet-Derived Growth Factor beta / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction


  • Receptor, Platelet-Derived Growth Factor beta