Efficacy of a combined protocol of urinary and recombinant follicle-stimulating hormone used for ovarian stimulation of patients undergoing ICSI cycle

J Assist Reprod Genet. 2007 Sep;24(9):400-5. doi: 10.1007/s10815-007-9159-0. Epub 2007 Jul 26.


Purpose: To evaluate the efficacy of using both urinary and recombinant FSH in a combined protocol for ovarian stimulation in an IVF treatment program.

Method: A total of 119 infertile couples undergoing ICSI treatment were randomized prospectively in this study. After a standard down-regulation with GnRH analogue, the patients were randomized in 2 groups 58 received combined urinary and recombinant FSH, starting with uFSH and then rFSH, and 61 controls received only recombinant FSH.

Result(s): Pregnancy and implantation rates were significantly higher in the combined uFSH/rFSH group than the control (rFSH) group (43.9% vs 22.1% and 27.5% vs 13.2% respectively). Metaphase II oocyte and grade 1 embryos were significantly higher in favour of combined uFSH/rFSH group than the recombinant FSH group.

Conclusion(s): This study shows that using a combination of both urinary and recombinant FSH for ovarian stimulation improves oocyte maturity and embryo cleavage, and increases pregnancy and implantation rates.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Drug Combinations
  • Embryo Implantation / drug effects
  • Female
  • Fertility Agents, Female / therapeutic use
  • Follicle Stimulating Hormone, Human / therapeutic use*
  • Humans
  • Infertility / drug therapy*
  • Male
  • Ovarian Hyperstimulation Syndrome / drug therapy*
  • Ovulation Induction / methods*
  • Pregnancy
  • Pregnancy Rate
  • Recombinant Proteins / therapeutic use
  • Sperm Injections, Intracytoplasmic*
  • Treatment Outcome
  • Urofollitropin / therapeutic use*


  • Drug Combinations
  • Fertility Agents, Female
  • Follicle Stimulating Hormone, Human
  • Recombinant Proteins
  • Urofollitropin