Tumor doubling time and local immune response to hepatic metastases from colorectal cancer

J Surg Oncol. 2007 Nov 1;96(6):525-33. doi: 10.1002/jso.20806.


Background and objectives: A number of studies have investigated the role of tumor-infiltrating lymphocytes in cancer, yet the local immune response to hepatic colorectal cancer metastasis remains unclear. As the tumor doubling time (DT) of hepatic colorectal cancer metastases is a good index of tumor growth, we examined the correlation between tumor DT and the local immune response by phenotype in hepatic colorectal cancer metastases.

Methods: Tumor DT and local immune response were examined in 20 patients with hepatic colorectal cancer metastases by analyzing tumor-infiltrating lymphocytes using flow cytometry or immunohistochemical studies. Tumor proliferative activity was also investigated by determining the expression levels of Ki-67 and proliferating cell nuclear antigen (PCNA).

Results: Locally abundant populations of CD83(+) dendritic cells (DCs) and CD8(+) T cells were positively related to longer tumor DT (P < 0.05), as were abundant CD8(+) T cells having interferon-gamma-producing potentials (P < 0.05). There was no significant correlation between tumor cell expression levels of Ki-67 or PCNA and tumor DT.

Conclusions: Longer DT tumors have increased local populations of CD8(+) T cells and CD83(+) DCs even in hepatic colorectal cancer metastases.

MeSH terms

  • Aged
  • Antigens, CD / metabolism
  • Cell Proliferation
  • Colorectal Neoplasms / pathology*
  • Dendritic Cells / immunology
  • Female
  • Humans
  • Immunoglobulins / metabolism
  • Ki-67 Antigen / metabolism
  • Liver Neoplasms / immunology*
  • Liver Neoplasms / pathology*
  • Liver Neoplasms / secondary
  • Lymphocytes, Tumor-Infiltrating / immunology*
  • Male
  • Membrane Glycoproteins / metabolism
  • Middle Aged
  • Proliferating Cell Nuclear Antigen / metabolism


  • Antigens, CD
  • CD83 antigen
  • Immunoglobulins
  • Ki-67 Antigen
  • Membrane Glycoproteins
  • Proliferating Cell Nuclear Antigen