Expression of livin in renal cell carcinoma and detection of anti-livin autoantibody in patients

Urology. 2007 Jul;70(1):38-42. doi: 10.1016/j.urology.2007.03.040.


Objectives: To assess the expression of livin, a member of the inhibitor of apoptosis protein family, in renal cell carcinoma (RCC) and to determine its prognostic relevance.

Methods: Immunohistochemical staining for livin was performed using paraffin-embedded tissues from 45 cases of RCC. Then we assessed anti-livin antibodies (Abs) in patient sera by enzyme-linked immunosorbent assay and Western blotting. Disease-specific survival of patients was assessed, and differences between the immunohistologically livin-positive and livin-negative groups and between the anti-livin Ab-positive and anti-livin Ab-negative groups were compared with recurrence-free survival using the Kaplan-Meier method and log-rank test.

Results: Of the 45 RCC specimens, 26 (57.8%) showed positive staining of livin immunohistochemically. In the RCC patients, anti-livin antibodies were detected and their levels were significantly higher than those in healthy volunteers. However, there was no difference in disease-specific survival between the livin-positive and livin-negative patients or between the anti-livin-positive and anti-livin-negative patients.

Conclusions: Although livin expression may not provide predictive information, it may be recognized as a tumor antigen by the immune system in RCC patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / biosynthesis*
  • Adaptor Proteins, Signal Transducing / immunology*
  • Adult
  • Aged
  • Aged, 80 and over
  • Autoantibodies / blood*
  • Carcinoma, Renal Cell / blood*
  • Carcinoma, Renal Cell / metabolism*
  • Female
  • Humans
  • Inhibitor of Apoptosis Proteins / biosynthesis*
  • Inhibitor of Apoptosis Proteins / immunology*
  • Kidney Neoplasms / blood*
  • Kidney Neoplasms / metabolism*
  • Male
  • Middle Aged
  • Neoplasm Proteins / biosynthesis*
  • Neoplasm Proteins / immunology*
  • Prognosis


  • Adaptor Proteins, Signal Transducing
  • Autoantibodies
  • BIRC7 protein, human
  • Inhibitor of Apoptosis Proteins
  • Neoplasm Proteins