Preparation of soluble extracts from adenovirus-infected cells for studies of RNA splicing

Oliver Mühlemann; Göran Akusjärvi

doi:10.1007/978-1-59745-277-9_3

Preparation of soluble extracts from adenovirus-infected cells for studies of RNA splicing

Methods Mol Med. 2007:131:33-46. doi: 10.1007/978-1-59745-277-9_3.

Authors

Oliver Mühlemann, Göran Akusjärvi

PMID: 17656773
DOI: 10.1007/978-1-59745-277-9_3

Abstract

Here we describe a collection of methods that have been adapted to produce highly efficient nuclear and cytoplasmic extracts from adenovirus-infected HeLa cells. We describe how to produce extracts from virus-infected cells and how to analyze RNA splicing in vitro using T7 RNA polymerase-derived splicing substrate RNAs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenoviridae / genetics*
HeLa Cells / virology
Humans
Polymerase Chain Reaction
RNA Splicing*
RNA, Messenger / genetics
Solubility

Substances

RNA, Messenger