In vitro methods to study RNA interference during an adenovirus infection

Methods Mol Med. 2007;131:47-61. doi: 10.1007/978-1-59745-277-9_4.

Abstract

RNA interference (RNAi) has attracted a lot of interest during recent years as a method to knock-down gene expression and as a possible antiviral system. Here we present a collection of in vitro methods to study RNAi and the effect of an adenovirus infection on RNAi. We describe methods to measure the two key enzymatic complexes involved in RNAi: Dicer and RISC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • HeLa Cells
  • Humans
  • RNA Interference*
  • RNA, Messenger / genetics
  • Ribonuclease III / metabolism

Substances

  • RNA, Messenger
  • Ribonuclease III