Transcription factor Pax5 activates the chromatin of key genes involved in B cell signaling, adhesion, migration, and immune function

Immunity. 2007 Jul;27(1):49-63. doi: 10.1016/j.immuni.2007.05.019. Epub 2007 Jul 19.


The transcription factor Pax5 represses B lineage-inappropriate genes and activates B cell-specific genes in B lymphocytes. Here we have identified 170 Pax5-activated genes. Conditional mutagenesis demonstrated that the Pax5-regulated genes require continuous Pax5 activity for normal expression in pro-B and mature B cells. Expression of half of the Pax5-activated genes is either absent or substantially reduced upon Pax5 loss in plasma cells. Direct Pax5 target genes were identified based on their protein synthesis-independent activation by a Pax5-estrogen receptor fusion protein. Chromatin immunoprecipitation (ChIP) of Pax5 together with chromatin profiling by ChIP-on-chip analysis demonstrated that Pax5 directly activates the chromatin at promoters or putative enhancers of Pax5 target genes. The Pax5-activated genes code for key regulatory and structural proteins involved in B cell signaling, adhesion, migration, antigen presentation, and germinal-center B cell formation, thus revealing a complex regulatory network that is activated by Pax5 to control B cell development and function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / metabolism*
  • Cell Adhesion / genetics
  • Cell Adhesion / immunology
  • Cell Differentiation / genetics
  • Cell Differentiation / immunology*
  • Cell Movement / genetics
  • Cell Movement / immunology*
  • Cells, Cultured
  • Chromatin / genetics
  • Chromatin / metabolism*
  • Gene Expression Profiling*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Oligonucleotide Array Sequence Analysis
  • PAX5 Transcription Factor / physiology*
  • Signal Transduction / genetics
  • Signal Transduction / immunology*
  • Stem Cells / cytology
  • Stem Cells / immunology
  • Stem Cells / metabolism


  • Chromatin
  • PAX5 Transcription Factor
  • Pax5 protein, mouse