Cooperation of two carotene desaturases in the production of lycopene in Myxococcus xanthus

FEBS J. 2007 Aug;274(16):4306-14. doi: 10.1111/j.1742-4658.2007.05960.x. Epub 2007 Jul 27.

Abstract

In Myxococcus xanthus, all known carotenogenic genes are grouped together in the gene cluster carB-carA, except for one, crtIb (previously named carC). We show here that the first three genes of the carB operon, crtE, crtIa, and crtB, encode a geranygeranyl synthase, a phytoene desaturase, and a phytoene synthase, respectively. We demonstrate also that CrtIa possesses cis-to-trans isomerase activity, and is able to dehydrogenate phytoene, producing phytofluene and zeta-carotene. Unlike the majority of CrtI-type phytoene desaturases, CrtIa is unable to perform the four dehydrogenation events involved in converting phytoene to lycopene. CrtIb, on the other hand, is incapable of dehydrogenating phytoene and lacks cis-to-trans isomerase activity. However, the presence of both CrtIa and CrtIb allows the completion of the four desaturation steps that convert phytoene to lycopene. Therefore, we report a unique mechanism where two distinct CrtI-type desaturases cooperate to carry out the four desaturation steps required for lycopene formation. In addition, we show that there is a difference in substrate recognition between the two desaturases; CrtIa dehydrogenates carotenes in the cis conformation, whereas CrtIb dehydrogenates carotenes in the trans conformation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkyl and Aryl Transferases / genetics
  • Alkyl and Aryl Transferases / metabolism
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Carotenoids / chemistry
  • Carotenoids / metabolism*
  • Geranylgeranyl-Diphosphate Geranylgeranyltransferase
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Lycopene
  • Models, Chemical
  • Molecular Structure
  • Myxococcus xanthus / enzymology
  • Myxococcus xanthus / genetics
  • Myxococcus xanthus / metabolism*
  • Operon
  • Oxidoreductases / genetics
  • Oxidoreductases / metabolism*
  • Spectrophotometry
  • Stereoisomerism
  • Substrate Specificity

Substances

  • Bacterial Proteins
  • Isoenzymes
  • Carotenoids
  • Oxidoreductases
  • phytoene dehydrogenase
  • Alkyl and Aryl Transferases
  • Geranylgeranyl-Diphosphate Geranylgeranyltransferase
  • Lycopene