Additive effects of CpG ODN and R-848 as adjuvants on augmenting immune responses to HBsAg vaccination

Biochem Biophys Res Commun. 2007 Sep 21;361(2):537-42. doi: 10.1016/j.bbrc.2007.07.028. Epub 2007 Jul 20.


In the present study, we tried to evaluate the ligands for Toll-like receptor 7 (TLR7) (R-848) and TLR 9 (CpG ODN) as adjuvants to augment the cellular and humoral immune responses as well as the generation of long-lasting immune memories following the vaccination with HBsAg in mice. The immune responses were assessed by enzyme-linked immunosorbent assay (ELISA), enzyme-linked immunospot (ELISPOT), and fluorescence-activated cell sorter (FACS) at the total and at the single-cell levels. Our results showed that CpG ODN or R-848 could enhance HBsAg-specific humoral and cellular immune responses following boosts. When R-848 in combination with CpG was used as adjuvants, the immune responses to HBsAg were further strengthened. Additional analysis demonstrated that the majority of the long-lasting HBsAg-specific T cells displayed effector memory phenotype. Taken together, our results imply that CpG ODN and R-848 may be the candidates as adjuvants for use in prophylactic and therapeutic hepatitis B vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • Antibodies, Viral / immunology
  • Antibody Formation / drug effects*
  • CD4-Positive T-Lymphocytes / drug effects
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / drug effects
  • CD8-Positive T-Lymphocytes / immunology
  • Drug Synergism
  • Female
  • Hepatitis B Surface Antigens / immunology*
  • Hepatitis B Vaccines / immunology*
  • Imidazoles / pharmacology*
  • Immunity, Cellular / drug effects*
  • Immunization, Secondary
  • Immunologic Memory / drug effects
  • Interferon-gamma / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Oligodeoxyribonucleotides / pharmacology*
  • Vaccination


  • Adjuvants, Immunologic
  • Antibodies, Viral
  • CPG-oligonucleotide
  • Hepatitis B Surface Antigens
  • Hepatitis B Vaccines
  • Imidazoles
  • Oligodeoxyribonucleotides
  • Interferon-gamma
  • resiquimod