Negamycin binds to the wall of the nascent chain exit tunnel of the 50S ribosomal subunit

Antimicrob Agents Chemother. 2007 Dec;51(12):4462-5. doi: 10.1128/AAC.00455-07. Epub 2007 Jul 30.

Abstract

Negamycin, a small-molecule inhibitor of protein synthesis, binds the Haloarcula marismortui 50S ribosomal subunit at a single site formed by highly conserved RNA nucleotides near the cytosolic end of the nascent chain exit tunnel. The mechanism of antibiotic action and the function of this unexplored tunnel region remain intriguingly elusive.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acids, Diamino / chemistry
  • Amino Acids, Diamino / metabolism
  • Amino Acids, Diamino / pharmacology
  • Binding Sites
  • Crystallography, X-Ray
  • Haloarcula marismortui / drug effects
  • Haloarcula marismortui / metabolism*
  • Models, Molecular
  • Molecular Structure
  • Ribosome Subunits, Large / chemistry
  • Ribosome Subunits, Large / metabolism*

Substances

  • Amino Acids, Diamino
  • negamycin