Lidocaine exhibits dose- and time-dependent cytotoxic effects on bovine articular chondrocytes in vitro

Am J Sports Med. 2007 Oct;35(10):1621-7. doi: 10.1177/0363546507304719. Epub 2007 Jul 30.


Background: Intra-articular lidocaine is commonly used.

Purpose: This study was conducted to determine whether short-term exposures to 1% and 2% lidocaine are toxic to articular chondrocytes, whether this is due to pH, and whether an intact articular surface is protective.

Study design: Controlled laboratory study.

Methods: Fresh bovine articular chondrocytes in alginate bead cultures were treated with 1% or 2% lidocaine or buffered saline (pH 7.4, 7.0, and 5.0) for 15, 30, or 60 minutes. Chondrocytes were then analyzed for viability by flow cytometry 1 hour, 1 day, and 1 week later. Bovine osteochondral cores with and without the superficial 1 mm of cartilage removed were submerged in either 0.9% saline (pH 7.4) or in 1% or 2% lidocaine for 30 minutes and assessed for viability using fluorescent microscopy.

Results: Chondrocyte viability decreased after just 15-minute exposures to 1% lidocaine. Longer exposures to 1% and 2% lidocaine further reduced chondrocyte viability. Chondrotoxicity of 2% lidocaine was greater than 1% lidocaine. There was no difference in chondrocyte viability after exposures to saline solutions of pH 7.4, 7.0, or 5.0. An intact articular surface did not affect lidocaine's chondrotoxic effects.

Conclusion: Results show dose- and time-dependent cytotoxic effects of lidocaine on bovine articular chondrocytes. Reduction of pH alone did not decrease chondrocyte viability, and the intact articular surface was not protective.

Clinical relevance: Although lidocaine chondrotoxicity was less than previously reported with bupivacaine, these observations suggest that local anesthetics as a class of drugs may negatively affect articular cartilage.

Publication types

  • Comparative Study

MeSH terms

  • Anesthetics, Local / pharmacology*
  • Animals
  • Cartilage, Articular / drug effects*
  • Cattle
  • Cell Death / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured
  • Chondrocytes / drug effects*
  • Dose-Response Relationship, Drug
  • Hydrogen-Ion Concentration
  • Lidocaine / pharmacology*
  • Sodium Chloride
  • Solutions
  • Time Factors


  • Anesthetics, Local
  • Solutions
  • Sodium Chloride
  • Lidocaine