Abstract
Ste4- mutants of Schizosaccharomyces pombe are unable to undergo both mating and meiosis. We have cloned the ste4+ gene and its cDNA. The gene encodes a 264 amino acid protein with a typical leucine zipper motif homologous with the jun family. However, unlike the jun family, this protein does not have a typical basic region that precedes the leucine zipper. The transcription of this gene absolutely depends on the ste11+ gene and increases several fold upon nitrogen starvation, a general signal for sexual differentiation. Whereas ste4+ is essential for mating and meiosis, its overexpression inhibits these processes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Cloning, Molecular
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GTP-Binding Protein beta Subunits*
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GTP-Binding Proteins / chemistry
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GTP-Binding Proteins / genetics*
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Gene Expression Regulation, Fungal / drug effects
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Gene Expression Regulation, Fungal / genetics
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Genes, Fungal / genetics*
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Genes, jun / genetics
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Genetic Complementation Test
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Heterotrimeric GTP-Binding Proteins*
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Leucine Zippers / genetics*
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Meiosis / genetics
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Molecular Sequence Data
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Mutation / genetics
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Nitrogen / pharmacology
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Proto-Oncogene Proteins c-jun / chemistry
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Proto-Oncogene Proteins c-jun / genetics
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Saccharomyces cerevisiae Proteins*
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Schizosaccharomyces / genetics*
Substances
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GTP-Binding Protein beta Subunits
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Proto-Oncogene Proteins c-jun
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Saccharomyces cerevisiae Proteins
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Ste4 protein, S cerevisiae
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GTP-Binding Proteins
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Heterotrimeric GTP-Binding Proteins
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Nitrogen
Associated data
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GENBANK/S74822
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GENBANK/S74824
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GENBANK/S74828
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GENBANK/S74831
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GENBANK/S74923
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GENBANK/S74924
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GENBANK/S74925
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GENBANK/S74926
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GENBANK/S75983
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GENBANK/X60103
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GENBANK/X61924