Delayed functional maturation of neonatal porcine islets in recipients under strict glycemic control

Xenotransplantation. 2007 Jul;14(4):333-8. doi: 10.1111/j.1399-3089.2007.00414.x.

Abstract

Background: The aim of this study was to compare the functional maturation of neonatal porcine islet (NPI) grafts exposed to long-term hyperglycemia with those implanted under euglycemic conditions.

Methods: mice Neonatal porcine islets were transplanted under the left renal capsule of diabetic SCID mice (group H), or in diabetic SCID mice who were also implanted with 500 BALB/c islets under the right renal capsule (group N). On day 42, the right kidneys were removed in both groups.

Results: No animals in group H achieved euglycemia within 3 weeks after transplantation. Thus, these mice were exposed to long-term hyperglycemia. Mice in group N became euglycemic immediately after transplantation, however after removal of BALB/c grafts on day 42 they exhibited significantly higher blood glucose levels than in group H and showed glucose intolerance after glucose administration. Cellular insulin content of NPI grafts harvested on day 58 or 72 was significantly lower in group N mice compared to group H.

Conclusions: These results suggest that tight control of glycemia reduces the functional maturation of NPI grafts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose / metabolism*
  • Delayed Graft Function / metabolism*
  • Diabetes Mellitus / therapy*
  • Disease Models, Animal
  • Glucose Tolerance Test
  • Hyperglycemia / metabolism
  • Islets of Langerhans Transplantation / methods
  • Islets of Langerhans Transplantation / physiology*
  • Male
  • Mice
  • Swine

Substances

  • Blood Glucose