Induction of interleukin-8 (CXCL-8) by tumor necrosis factor-alpha and leukemia inhibitory factor in pancreatic carcinoma cells: Impact of CXCL-8 as an autocrine growth factor

Int J Oncol. 2007 Sep;31(3):627-32.


Pancreatic carcinoma is one of the most lethal of the gastrointestinal malignant tumors. Chronic inflammation leads to cancer development and progression. Interleukin-8 (CXCL-8) is a CXC chemokine, which plays an important role in neutrophil chemotaxis and activation. We previously reported that CXCL-8 was produced by a variety of human carcinoma cells and tissues, and that CXCL-8 promoted proliferation in pancreatic carcinoma cells (SUIT-2). In the present study, we analyzed whether various cytokines affect cell proliferation by CXCL-8 expression in pancreas carcinoma cells. All examined pancreatic carcinoma cells expressed CXCL-8 and TNFRII mRNA constitutively in RPMI-1640 medium without FBS. TNF-alpha, LIF, IL-1beta, IL-6, IL-8, or IFN-beta enhanced the expression of CXCL-8 mRNA, but IL-10 did not in Hs-700T cells. Actinomycin D suppressed and cycloheximide augmented CXCL-8 mRNA which was induced by TNF-alpha or not. The half-life of CXCL-8 mRNA was 36.5 min by TNF-alpha and 35.2 min by no stimulation. In our previous study, LIF promoted cell growth in Hs-700T cells. LIF induced CXCL-8 mRNA in a dose- and time-dependent manner. Addition of recombinant CXCL-8 did not induce cell growth of Hs-700T. Anti-CXCL-8 IgG significantly suppressed cell growth. CXCL-8 would act as an autocrine growth factor in Hs-700T cells, which expressed CXCL-8 mRNA highly without stimulation. Curcumin (diferuloylmethane), NF-kappaB inhibitor, suppressed cell proliferation in Hs-700T cells. These results suggest that CXCL-8 plays a pivotal role in progression of pancreatic cancer, and its expression is influenced by inflammatory cytokines in pancreatic tumor microenvironment.

MeSH terms

  • Cell Line, Tumor
  • Cell Proliferation
  • Cycloheximide / pharmacology
  • Cytokines / metabolism
  • Dactinomycin / pharmacology
  • Humans
  • Immunoglobulin G / chemistry
  • Inflammation
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Interleukin-8 / metabolism*
  • Leukemia Inhibitory Factor / metabolism*
  • Pancreatic Neoplasms / metabolism*
  • RNA, Messenger / metabolism
  • Time Factors
  • Tumor Necrosis Factor-alpha / metabolism*


  • Cytokines
  • Immunoglobulin G
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-8
  • Leukemia Inhibitory Factor
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Dactinomycin
  • Cycloheximide