Objective: To determine the content of bergenin in Ardisia pusilla.
Methods: High Performance Liquid Chromatography was performed on Hyersil C18ODS. The chromatorgraphic conditions were as follows: methanol-water (20:80) as mobile phase, flow rate being 0.600 ml/min and detecting wavelength at 275 nm. The column temperature was 25 degrees C.
Results: A good linearity of bergenin was shown in range of 0. 098-0. 591 microg (r = 0.9998), the average recovery is 102.0%, RSD = 1.4% (n=6). The content of bergenin in Ardisia pusilla is 59% at least.
Conclusion: The content of bergenin in Ardisia pusilla and A. japonica is identical.