Cryopreservation of granule cells from the postnatal rat hippocampus

J Pharmacol Sci. 2007 Aug;104(4):387-91. doi: 10.1254/jphs.sc0070162. Epub 2007 Aug 4.

Abstract

Although primary cultures of neurons are essential methods for cell biological and pharmacological researches, many animals must be sacrificed for each experiment. Here we introduce a novel system to cryopreserve hippocampal granule cells (GCs) prepared from postnatal rats. Being thawed after as long as 60 days of cryopreservation, GCs expressed the mature neuronal marker MAP-2 and elongated single tau-1-positive axons and multiple tau-1-negative dendrites. These properties closely resembled intact GCs in primary cultures, providing the advantage of being able to repeatedly prepare stable cultures with a single sacrifice of animals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / analysis
  • Axons / metabolism
  • Cells, Cultured
  • Cryopreservation / methods*
  • Dendrites / metabolism
  • Gene Expression
  • Hippocampus / cytology*
  • Microtubule-Associated Proteins / metabolism
  • Rats
  • Rats, Wistar
  • Time Factors

Substances

  • Antibodies, Monoclonal
  • MAP2 protein, rat
  • Microtubule-Associated Proteins
  • tau-1 monoclonal antibody