We have studied the performance of polytetrafluoroethylene integrating spheres in the ultraviolet (UV) region with wavelengths as short as 200 nm. Two techniques were used for this study; first, the spectral throughput of an integrating sphere irradiated by a deuterium lamp was analyzed by a monochromator. Second, a UV laser beam was directed into an integrating sphere, and spectrally dispersed laser induced fluorescence was studied. Significant absorption and fluorescence features were observed in the UV region and attributed to the contamination in the integrating sphere. We demonstrate that integrating spheres are easily contaminated by environmental pollutants such as polycyclic aromatic hydrocarbons emitted from engine exhaust. Baking of the contaminated integrating sphere can reverse some but not all of the effects caused by contaminants. The implications for using integrating spheres for UV measurement are discussed.