Measurements of the hysteresis in unzipping and rezipping double-stranded DNA

Phys Rev E Stat Nonlin Soft Matter Phys. 2007 May;75(5 Pt 1):051908. doi: 10.1103/PhysRevE.75.051908. Epub 2007 May 11.

Abstract

Complete unzipping and rezipping of lambda -phage double-stranded DNA is achieved by applying a constant force. A strong hysteresis is observed at all tested time scales and temperatures. Hysteresis also occurs for partial unzipping, indicating stability for the partially open state over a force range of 2- 5pN . Results are compared to nearest-neighbor model simulations, and reasonable agreement is found.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacteriophage lambda / genetics*
  • Computer Simulation
  • DNA, Viral / chemistry*
  • DNA, Viral / ultrastructure*
  • Models, Chemical*
  • Models, Molecular*
  • Nonlinear Dynamics
  • Nucleic Acid Conformation
  • Nucleic Acid Denaturation*
  • Stress, Mechanical

Substances

  • DNA, Viral