Bone morphogenetic protein signaling is essential for terminal differentiation of the intestinal secretory cell lineage

Gastroenterology. 2007 Sep;133(3):887-96. doi: 10.1053/j.gastro.2007.06.066. Epub 2007 Jul 3.


Background & aims: Bone morphogenetic proteins (Bmps) are morphogens known to play key roles in gastrointestinal development and pathology. Most Bmps are produced primarily by the mesenchymal compartment and activate their signaling pathways following a paracrine or autocrine route. The aim of this study was to investigate the role of epithelial Bmp signaling in intestinal morphogenesis and maintenance of adult epithelial cell functions.

Methods: With the use of tissue-specific gene ablation, we generated mice lacking the Bmp receptor type IA (Bmpr1a) exclusively in the intestinal epithelium. Bmpr1a mutant and control mice were sacrificed for histology, immunofluorescence, Western blot analysis, electron microscopy, and quantitative polymerase chain reaction.

Results: As well as showing increased proliferation and altered intestinal epithelial morphology, Bmpr1a mutant mice revealed that epithelial Bmp signaling is associated with impaired terminal differentiation of cells from the secretory lineage but not with the determination of cell fate. Loss of Bmp signaling exclusively in the epithelial compartment is not sufficient for the initiation of the de novo crypt phenomenon associated with juvenile polyposis syndrome.

Conclusions: Epithelial Bmp signaling plays an important role in the terminal differentiation of the intestinal secretory cell lineage but not in de novo crypt formation. These findings emphasize the importance of delineating the contribution of the stroma vs the epithelium in gastrointestinal physiology and pathology.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Morphogenetic Protein Receptors, Type I / genetics
  • Bone Morphogenetic Protein Receptors, Type I / metabolism
  • Bone Morphogenetic Proteins / genetics
  • Bone Morphogenetic Proteins / metabolism*
  • Cell Differentiation / physiology*
  • Cell Proliferation
  • Intestinal Mucosa / cytology
  • Intestinal Mucosa / metabolism
  • Jejunum / cytology*
  • Jejunum / metabolism*
  • Jejunum / pathology
  • Mice
  • Mice, Inbred Strains
  • Mice, Transgenic
  • Mutation / genetics
  • Signal Transduction / physiology*
  • Wnt Proteins / physiology
  • beta Catenin / physiology


  • Bone Morphogenetic Proteins
  • Wnt Proteins
  • beta Catenin
  • Bmpr1a protein, mouse
  • Bone Morphogenetic Protein Receptors, Type I