Fate of 2,2,2-trichloroacetaldehyde (chloral hydrate) produced during trichloroethylene oxidation by methanotrophs

Appl Environ Microbiol. 1991 Aug;57(8):2399-402. doi: 10.1128/aem.57.8.2399-2402.1991.


Four different methanotrophs expressing soluble methane monooxygenase produced 2,2,2-trichloroacetaldehyde, or chloral hydrate, a controlled substance, during the oxidation of trichloroethylene. Chloral hydrate concentrations decreased in these cultures between 1 h and 24 h of incubation. Chloral hydrate was shown to be biologically transformed to trichloroethanol and trichloroacetic acid by Methylosinus trichosporium OB3b. At elevated pH and temperature, chloral hydrate readily decomposed and chloroform and formic acid were detected as products.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chloral Hydrate / metabolism*
  • Methylococcaceae / enzymology*
  • Oxidation-Reduction
  • Oxygenases / metabolism
  • Trichloroethylene / metabolism*


  • Trichloroethylene
  • Chloral Hydrate
  • Oxygenases
  • methane monooxygenase