Rab6 regulates transport and targeting of exocytotic carriers

Dev Cell. 2007 Aug;13(2):305-14. doi: 10.1016/j.devcel.2007.06.010.

Abstract

Constitutive exocytosis delivers newly synthesized proteins, lipids, and other molecules from the Golgi apparatus to the cell surface. This process is mediated by vesicles, which bud off the trans-Golgi network, move along cytoskeletal filaments, and fuse with the plasma membrane. Here, we show that the small GTPase Rab6 marks exocytotic vesicles and, together with the microtubule plus-end-directed motor kinesin-1, stimulates their processive microtubule-based transport to the cell periphery. Furthermore, Rab6 directs targeting of secretory vesicles to plasma-membrane sites enriched in the cortical protein ELKS, a known Rab6 binding partner. Our data demonstrate that although Rab6 is not essential for secretion, it controls the organization of exocytosis within the cellular space.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / metabolism
  • Animals
  • Biological Transport
  • Biomarkers / metabolism
  • Cell Line
  • Cell Membrane / metabolism
  • Cytoplasm / metabolism
  • Dogs
  • Dyneins / metabolism
  • Exocytosis*
  • HeLa Cells
  • Humans
  • Kinesin / metabolism
  • Membrane Fusion
  • Nerve Tissue Proteins / metabolism
  • Rats
  • Transport Vesicles / metabolism*
  • rab GTP-Binding Proteins / metabolism*

Substances

  • Adaptor Proteins, Signal Transducing
  • Biomarkers
  • ERC1 protein, human
  • Nerve Tissue Proteins
  • Rab6 protein
  • Dyneins
  • Kinesin
  • rab GTP-Binding Proteins