Identification of astaxanthin diglucoside diesters from snow alga Chlamydomonas nivalis by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry

Phytochemistry. 2008 Jan;69(2):479-90. doi: 10.1016/j.phytochem.2007.06.025. Epub 2007 Aug 2.


A method is described for the identification of astaxanthin glucoside esters from snow alga Chlamydomonas nivalis by means of liquid chromatography-mass spectrometry with atmospheric pressure chemical ionization (LC-MS/APCI). The method is based on the use of preparative HPLC and subsequent identification of astaxanthin diglucoside diesters by microbore LC-MS/APCI. The combination of these two techniques was used to identify more than 100 molecular species. The astaxanthin diglucoside diester, i.e. (all-E)-[di-(6-O-oleoyl-beta-D-glucopyranosyloxy)]-astaxanthin, was also synthesized to unambiguously confirm its structure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chlamydomonas / chemistry*
  • Chromatography, Liquid
  • Glucosides / chemistry*
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Pressure
  • Snow
  • Spectrometry, Mass, Electrospray Ionization
  • Xanthophylls / chemistry
  • Xanthophylls / isolation & purification


  • Glucosides
  • Xanthophylls
  • astaxanthine