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, 70 (3), 557-60

A Simple In-Vitro 'Wet-Plate' Method for Mass Production of Phytophthora Nicotianae Zoospores and Factors Influencing Zoospore Production

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A Simple In-Vitro 'Wet-Plate' Method for Mass Production of Phytophthora Nicotianae Zoospores and Factors Influencing Zoospore Production

Monday O Ahonsi et al. J Microbiol Methods.

Abstract

A simple in-vitro 'wet-plate' method for mass-producing Phytophthora nicotianae zoospores at > or =1.0 x 10(6) zoospores/ml is described. Temperature critically affected zoospore production; 22 degrees C was optimum, while 36 degrees C was completely inhibitory. Zoospores being the most important propagule of P. nicotianae, temperature of recycled irrigation water may be manipulated to reduce diseases in irrigated nursery crops.

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