Increased production of lipocalin-type prostaglandin D synthase in leptomeningeal cells through contact with astrocytes

Neurosci Lett. 2007 Aug 16;423(2):133-7. doi: 10.1016/j.neulet.2007.06.045. Epub 2007 Aug 1.


Lipocalin-type prostaglandin D synthase (L-PGDS) is dominantly expressed in the leptomeninges surrounding the brain and secreted into the cerebrospinal fluid as beta-trace, a major cerebrospinal fluid protein. To examine the interaction between the leptomeninges and the brain parenchyma, we co-cultured rat leptomeningeal cells with cells dissociated from the neonatal rat cortex and found that the production of L-PGDS was remarkably increased after the co-cultivation. A similar increase in L-PGDS production was observed by the co-culturing of the leptomeningeal cells with cells dissociated from astrocyte-rich cultures or with 1321-N1 astrocytoma cells. When a crude membrane fraction prepared from 1321-N1 cells was added to leptomeningeal cell cultures, L-PGDS gene expression was slowly increased up to 48 h after the addition. These results indicate that leptomeningeal cells enhance their L-PGDS production by a slow activation of L-PGDS gene expression through their contact with astrocytes.

MeSH terms

  • Animals
  • Animals, Newborn
  • Astrocytes / metabolism*
  • Cell Communication / physiology
  • Cell Line, Tumor
  • Cells, Cultured
  • Coculture Techniques
  • Gene Expression
  • Gene Expression Regulation, Enzymologic*
  • Humans
  • Intramolecular Oxidoreductases / biosynthesis*
  • Lipocalins
  • Meninges / cytology*
  • Meninges / enzymology*
  • RNA, Messenger / analysis
  • Rats
  • Rats, Wistar
  • Reverse Transcriptase Polymerase Chain Reaction


  • Lipocalins
  • RNA, Messenger
  • Intramolecular Oxidoreductases
  • prostaglandin R2 D-isomerase