Structure of the O-antigen of Francisella Tularensis Strain 15

Carbohydr Res. 1991 Jul 30;214(2):289-97. doi: 10.1016/0008-6215(91)80036-m.

Abstract

The O-specific polysaccharide, obtained by mild acid degradation of the lipopolysaccharide of Francisella tularensis strain 15, contained 2-acetamido-2,6-dideoxy-D-glucose (D-QuiNAc), 4,6-dideoxy-4-formamido-D-glucose (D-Qui4NFm), and 2-acetamido-2-deoxy-D-galacturonamide (D-GalNAcAN) in the ratios 1:1:2. Tri- and tetra-saccharide fragments were obtained on treatment of the polysaccharide with anhydrous hydrogen fluoride and partial hydrolysis with 0.1 M hydrochloric acid, respectively. On the basis of 1H- and 13C-n.m.r. spectroscopy of the polysaccharide and the saccharides, it was concluded that the O-antigen had the structure: ----4)-alpha-D-GalpNAcAN-(1----4)-alpha-D-GalpNAcAN-(1----3) -beta-D-QuipNAc-(1----2)-beta-D-Quip4NFm-(1----. This O-antigen is related in structure to those of Pseudomonas aeruginosa O6, immunotype 1, and IID 1008, and Shigella dysenteriae type 7.

Publication types

  • Comparative Study

MeSH terms

  • Antigens, Bacterial / chemistry*
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Francisella tularensis / chemistry
  • Francisella tularensis / immunology*
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Polysaccharides, Bacterial / chemistry
  • Polysaccharides, Bacterial / immunology
  • Pseudomonas aeruginosa / chemistry
  • Pseudomonas aeruginosa / immunology
  • Shigella dysenteriae / chemistry
  • Shigella dysenteriae / immunology
  • Species Specificity

Substances

  • Antigens, Bacterial
  • Polysaccharides, Bacterial