Tomographic phase microscopy

Nat Methods. 2007 Sep;4(9):717-9. doi: 10.1038/nmeth1078. Epub 2007 Aug 12.


We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / ultrastructure
  • HeLa Cells
  • Humans
  • Imaging, Three-Dimensional* / instrumentation
  • Imaging, Three-Dimensional* / methods
  • Microscopy, Phase-Contrast* / instrumentation
  • Tomography* / instrumentation
  • Tomography* / methods